For UV detection of colorless substances, plates with two kinds of fluorescent indicator are available: green fluorescing F254 (manganese activated zinc silicate) or blue fluorescing F254s (magnesium wolframate).
In addition, F254s is highly stable in acidic solvent systems. Both indicators fluorescence in UV light at an excitation wavelength of 254 nm.
Samples which absorb short-wave UV at 254 nm are detected due to fluorescence quenching.
Specially developed high-fluorescence LuxPlates contain a higher content of fluorescent indicator for further improved identification of separate zones.
Classic TLC silica plates, GF254 (Silica gel with gypsum as binding agent, fluorescence background at 254nm), 60 Å pore size. Aluminum backed-easily cut by a pair of scissors.
Precut size of 2.5cm by 7.5cm, 80 plates per box.
What's ideal is that these plates have neutral silica (pH 6-7) instead of the more common acidic silica (pH=5) for TLC silica plates. The neutral silica make the plates compatible with running compounds with an amino group even without the need of adding triethylamine. At the same time non-amino compounds behave the same as on acidic TLC plates.
Actual examples performed with the plates have been uploaded for users to see.
Classic TLC silica plates, GF254 (Silica gel with gypsum as binding agent, fluorescence background at 254nm), 60 Å pore size. Aluminum backed-easily cut by a pair of scissors.
20 plates per box, 10cm x 10cm size.
What's ideal is that these plates have neutral silica (pH 6-7) instead of the more common acidic silica (pH=5) for TLC silica plates. The neutral silica make the plates compatible with running compounds with an amino group even without the need of adding triethylamine. At the same time non-amino compounds behave the same as on acidic TLC plates.
Actual examples performed with the plates have been uploaded for users to see.
Classic TLC silica plates, GF254 type (fluorescence background at 254nm), 60 Å pore size. Aluminum backed-easily cut by a pair of scissors.
24 plates per box, 10cm x 20cm size.
What's ideal is that these plates have neutral silica (pH 6-7) instead of the more common acidic silica (pH=5) for TLC silica plates. The neutral silica make the plates compatible with running compounds with an amino group even without the need of adding triethylamine. At the same time non-amino compounds behave the same as on acidic TLC plates.
Actual examples performed with the plates have been uploaded for users to see.
For UV detection of colorless substances, plates with two kinds of fluorescent indicator are available: green fluorescing F254 (manganese activated zinc silicate) or blue fluorescing F254s (magnesium wolframate).
In addition, F254s is highly stable in acidic solvent systems. Both indicators fluorescence in UV light at an excitation wavelength of 254 nm.
Samples which absorb short-wave UV at 254 nm are detected due to fluorescence quenching.
Specially developed high-fluorescence LuxPlates contain a higher content of fluorescent indicator for further improved identification of separate zones.
1. You may cut glass plates of up to 20 cm wide, and of any length.
2. The white measuring plate can be removed, if your TLC plate happens to be too thick to fit (usually when cutting preparative TLC plates).
3. The side screw fixed onto a turning-knob is for controlling the stop position of the scribbler, so that one can prevent the scribbler from cutting off of the glass plate and chipping off a small piece.
4. Usually, start from the far side relative to the screw, and cut towards the screw.
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